Journal: HardwareX

Article Title: Microscope Upcycling: Transforming legacy microscopes into automated cloud-integrated imaging systems

doi: 10.1016/j.ohx.2025.e00637

Figure Lengend Snippet: (a) Automated microscopy setup. (1) Microscope camera connected to computer (not visible). (2) Motorized XY stage composed of 2 NEMA 11 linear rail actuators coupled with metal and plastic parts. (3) Rectangular plate holding attachment for 6,12,24, and 96 well plates. (4) Bent steel plate used to hold stepper motor in place, attached to table surface with double sided tape. (5) StepperOnline 17HS08-1004S stepper motor for controlling focus. (6) 3 mm to 5 mm flexible shaft coupling for attaching stepper motor to microscope focus wheel. (b) Visual reference for focus controller assembly guide. (a) The fine focus knob of the Zeiss Axiovert 25 microscope which is removed in Section item 2. (b) The interface between the stepper motor of the Z focus module and the mounting bracket, which is described in Section item 1. (c) The coupling that attaches the stepper motor of the Z focus module to the focus controller of the Zeiss Axiovert 25 Microscope. This is listed in the Bill of Materials as Z axis coupling, and is used in Section item 3.

Article Snippet: Closest commercial analog , Thorlabs MLS203-1 Motorized XY Scanning Stage and Thorlabs MFC1 Motorized Microscope Focus Controller.

Techniques: Microscopy

Journal: HardwareX

Article Title: Microscope Upcycling: Transforming legacy microscopes into automated cloud-integrated imaging systems

doi: 10.1016/j.ohx.2025.e00637

Figure Lengend Snippet: (a) Isometric rendering of the XY stage created in Fusion 360. (b) Isometric rendering of the focus controller. (c) Disassembled components for motorized XY stage. (1) X axis linear rail which control motion along the X axis. (2) Y axis linear rail which controls motion along the Y axis. (3) Primary Motor Bracket which attaches and squares up the X axis to the right side of the imaging surface of the microscope. (4) Secondary Motor Bracket which attaches and squares up the X axis to the left side of the imaging surface of the microscope. (5) Motor Pairing Plate which allows for attaching the Y axis motor to the X axis motor. (6) Motor Mounting Spacer which allows parts to be attached to the X and Y axis motors without causing collisions with the ends of the motor rails. (7) Plate Holder for holding standard cell culture well plates (i.e. 6, 12, 24, 96, or 384 well plates).

Article Snippet: Closest commercial analog , Thorlabs MLS203-1 Motorized XY Scanning Stage and Thorlabs MFC1 Motorized Microscope Focus Controller.

Techniques: Control, Imaging, Microscopy, Cell Culture

Journal: HardwareX

Article Title: Microscope Upcycling: Transforming legacy microscopes into automated cloud-integrated imaging systems

doi: 10.1016/j.ohx.2025.e00637

Figure Lengend Snippet:

Article Snippet: Closest commercial analog , Thorlabs MLS203-1 Motorized XY Scanning Stage and Thorlabs MFC1 Motorized Microscope Focus Controller.

Techniques: Imaging, Microscopy

Journal: HardwareX

Article Title: Microscope Upcycling: Transforming legacy microscopes into automated cloud-integrated imaging systems

doi: 10.1016/j.ohx.2025.e00637

Figure Lengend Snippet:

Article Snippet: Closest commercial analog , Thorlabs MLS203-1 Motorized XY Scanning Stage and Thorlabs MFC1 Motorized Microscope Focus Controller.

Techniques: Control, Microscopy